Identification ofXanthomonas citrissp.citrihost specificity genes in a heterologous expression host
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چکیده
منابع مشابه
A Streptomyces coelicolor host for the heterologous expression of Type III polyketide synthase genes
BACKGROUND Recent advances in genome sequencing, combined with bioinformatic analysis, has led to the identification of numerous novel natural product gene clusters, particularly in actinomycetes of terrestrial and marine origin. Many of these gene clusters encode uncharacterised Type III polyketide synthases. To facilitate the study of these genes and their potentially novel products, we set o...
متن کاملAllelic and Nonallelic Genes Controlling Host Specificity in a Bacteriophage.
ACTERIA infected with any two independently arising r (plaque type) B mutants of the bacterial virus T2H have been found, as a rule, to yield wild-type and double-mutant individuals among the viral progeny ( HERSHEY and ROTMAN 1948). This result has been interpreted in terms of recombination among nonallelic genetic-factors, and it is permissible to speak of the test in which bacteria are infec...
متن کاملIdentification and heterologous expression of genes involved in anaerobic dissimilatory phosphite oxidation by Desulfotignum phosphitoxidans.
Desulfotignum phosphitoxidans is a strictly anaerobic, Gram-negative bacterium that utilizes phosphite as the sole electron source for homoacetogenic CO2 reduction or sulfate reduction. A genomic library of D. phosphitoxidans, constructed using the fosmid vector pJK050, was screened for clones harboring the genes involved in phosphite oxidation via PCR using primers developed based on the amino...
متن کاملHeterologous Expression of Bovine Prochymosin in Pichia pastoris GS115
Objectives: In present research we evaluate the expression of this critical enzyme in a eukaryotic system for future use in cheese industry. Materials and Methods: We have cloned bovine prochymosin gene in methylotrophic yeast, P. pastoris, using pPIC9K as an expression vector. The recombinant plasmid was transformed into the host by electroporation, and it was expre...
متن کاملHeterologous expression of two trichothecene P450 genes in Fusarium verticillioides.
Fusarium graminearum Z-3639 and F. sporotrichioides NRRL3299 produce the trichothecene mycotoxins 15-acetyldeoxynivalenol and T-2 toxin, respectively. These toxins differ in oxygenation at C-4, C-7, and C-8. In F. sporotrichioides, Tri1 (FsTri1) controls C-8 hydroxylation. To determine the function of an apparent F. graminearum Tri1 (FgTri1) homolog, both FsTri1 and FgTri1 genes were heterologo...
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ژورنال
عنوان ژورنال: Molecular Plant Pathology
سال: 2009
ISSN: 1464-6722,1364-3703
DOI: 10.1111/j.1364-3703.2008.00528.x